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鍖栧铔嬬櫧璐ㄧ粍瀛︽槸娉ㄩ噴铔嬬櫧璐ㄥ姛鑳藉拰鍙戠幇鐢熺墿娲绘�ч厤浣撻澏鏍囩殑閲嶈宸ュ叿銆傚湪鍖栧铔嬬櫧璐ㄧ粍瀛︽妧鏈腑锛屾渶甯哥敤鐨勬槸鍩轰簬娲绘�х殑铔嬬櫧璐ㄥ垎鏋愶紙Activity-based protein profiling锛岀畝绉癆BPP锛?/span>锛岃鏂规硶閫氳繃缁撳悎娲绘�у垎瀛愭帰閽?/span>鏍囪鍜屼覆鑱旇川璋卞垎鏋?/span>锛?/span>瀹炵幇闈惰泲鐧界殑閴村畾銆?/span>闅忕潃瀹氶噺缁勫鎶�鏈殑鍙戝睍锛岀瀛﹀浠皢娲绘�у垎瀛愭帰閽堝拰鍩轰簬鍚屼綅绱犳爣绛剧殑铔嬬櫧璐ㄧ粍瀛︽柟娉曠浉缁撳悎锛屽紑鍙戜簡涓�绯诲垪瀹氶噺鍖栧铔嬬櫧璐ㄧ粍瀛︽妧鏈紝鍖呮嫭鍩轰簬涓�绾ц氨瀹氶噺鐨?/span>isoTOP锛堝熀浜庡悓浣嶇礌鐨勪覆鑱旀浜よ泲鐧芥按瑙ｏ級-ABPP鎶�鏈拰rdTOP锛堝熀浜庤繕鍘熶簩鐢插熀鍖栫殑涓茶仈姝ｄ氦铔嬬櫧姘磋В锛?ABPP鎶�鏈互鍙婂熀浜庝簩绾ц氨瀹氶噺鐨凪TRP锛堝閫氶亾娲绘�у矾鍩虹瓫閫夛級鎶�鏈拰SLC锛堟祦绾垮瀷鐨勫崐鑳辨皑閰革級-ABPP鎶�鏈?/span>銆傜劧鑰岋紝杩欎簺鎶�鏈緷璧栦簬鍗婇殢鏈烘�х殑鏁版嵁渚濊禆鍨嬭川璋遍噰闆嗘ā寮忥紙Data-dependent acquisition锛岀畝绉癉DA锛夛紝浠ュ強鎴愭湰楂樻槀鐨勫悓浣嶇礌瀹氶噺鏍囩璇曞墏锛岄檺鍒朵簡鍖栧铔嬬櫧璐ㄧ粍瀛︽妧鏈湪澶氱淮鍒嗘瀽鍜岄珮閫氶噺绛涢�変腑鐨勫簲鐢ㄣ�?/span>
浼撮殢鐫�楂樼伒鏁忓害銆侀珮鍒嗚鲸鐜囥�侀珮绮惧害鐨勮川璋变华鍣ㄧ殑蹇�熸洿鏂帮紝浠ュ強鐢熺墿淇℃伅瀛﹀浜庢捣閲忔暟鎹鐞嗚兘鍔涚殑涓嶆柇鎻愰珮锛岃繎浜涘勾鏉ュ叴璧风殑鍩轰簬鏁版嵁闈炰緷璧栧瀷閲囬泦鐨勮川璋遍噰闆嗘ā寮廌ata-independent acquisition锛岀畝绉癉IA锛?/span>閫愭笎鍦ㄨ泲鐧借川缁勫鐮旂┒棰嗗煙涓?/span>宕湶澶磋銆?/span>涓庝紶缁熺殑DDA妯″紡涓嶅悓锛?/span>DIA妯″紡骞朵笉浠呴檺浜庡楂樺己搴︾殑鑲芥绂诲瓙杩涜閲囬泦鍜屽垎鏋愶紝鑰屾槸灏嗙瀛愭牴鎹?/span>璐ㄨ嵎姣斿ぇ灏忓垝鍒?/span>鎴愯嫢骞蹭釜閲囬泦绐楀彛鍚?/span>锛屽姣忎釜绐楀彛涓墍鏈夎偨娈佃繘琛屽悎骞跺垎鏋愶紝浠庤�屽彲浠ュ疄鐜版棤閬楁紡銆佹棤宸紓鐨勮偨娈典俊鎭噰闆嗭紝鍥犳锛孌IA妯″紡鍏锋湁鏇村ソ鐨勮鐩栧害锛岄噸鐜版�т互鍙婂畾閲忓噯纭�с�?/span>
杩戞棩锛屽寳浜ぇ瀛﹀寲瀛︿笌鍒嗗瓙宸ョ▼瀛﹂櫌銆佸寳澶?/span>-娓呭崕鐢熷懡绉戝鑱斿悎涓績鐜嬪垵璇鹃缁勫湪Journal of the American Chemical Society鏉傚織涓婂彂琛ㄤ簡棰樹负鈥淨uantitative Chemoproteomic Profiling with Data-Independent Acquisition-based Mass Spectrometry鈥濈殑鐮旂┒璁烘枃銆傚湪杩欓」宸ヤ綔涓紝浣滆�呭皢DIA妯″紡涓?/span>ABPP鎶�鏈?/span>鐩哥粨鍚堬紝鍙戝睍浜嗗悕涓篋IA-ABPP鐨?/span>鏃犳爣瀹氶噺鏂规硶銆傝鏂规硶瑙ｅ喅浜?/span>涔嬪墠瀹氶噺鎶�鏈?/span>涓?/span>鐢盌DA妯″紡寮曡捣鐨勬暟鎹涪澶变弗閲嶄互鍙婇珮鎴愭湰鐨?/span>鍚屼綅绱犳爣绛捐瘯鍓?/span>鐨勪娇鐢ㄧ瓑闂锛屾槸涓�绉嶈鐩栧害楂?/span>銆侀噸鐜版�?/span>濂?/span>浠ュ強瀹氶噺鍑嗙‘鐨?/span>鍖栧铔嬬櫧璐ㄧ粍瀛︽柊绛栫暐銆?/span>
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姝ゅ锛屼綔鑰呬篃灏嗘柊鏂规硶鐢ㄤ簬鍏变环鐗囨灏忓垎瀛愬簱鐨勭瓫閫夈�?/span>浣滆�呮瀯寤轰簡24涓叡浠风墖娈靛垎瀛愬簱杩涜鍒嗘瀽锛屽彂鐜颁簡563涓彲闈跺悜鐨?/span>铔嬬櫧璐?/span>銆傚浜庡崟涓�铔嬬櫧鑰岃█锛屾柊鏂规硶鍙互閴村畾鍒版洿澶氱殑鍗婅儽姘ㄩ吀浣嶇偣锛屼緥濡傛牳瀛旇泲鐧絅UP205锛孌IA-ABPP鏂规硶鍙互瀹氶噺16涓崐鑳辨皑閰革紝鑰屽師鏉ョ殑鏂规硶鍙兘鑾峰緱2涓崐鑳辨皑閰哥殑瀹氶噺淇℃伅銆傛澶栵紝浣滆�呬篃瀵瑰叿鏈夌浉鍚岀粨鍚堝熀鍥紝涓嶅悓鍙嶅簲鍩哄洟鐨勭墖娈靛垎瀛愬杩涜浜嗗ご瀵瑰ご鐨勬瘮杈冿紝寮鸿皟浜嗗弽搴斿熀鍥㈠鍗婅儽姘ㄩ吀闈跺悜鎬х殑褰卞搷銆?/span>
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//pubs.acs.org/doi/pdf/10.1021/jacs.1c11053