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浣滀负绁炵粡绯荤粺淇℃伅浜ゆ祦鐨勨�滈�氳揣鈥濓紝绁?/span>缁?/span>鐢垫椿鍔ㄦ槸澶ц剳澶勭悊澶嶆潅淇℃伅鐨?/span>鐗╃悊鍩虹銆?/span>涓?/span>鑶滅墖閽冲拰寰數鏋侀樀鍒?/span>璁板綍绛?/span>鍩轰簬鐢垫瀬鏉愭枡鐨?/span>浼犵粺鐢电敓鐞嗘妧鏈?/span>鐩告瘮锛?/span>鑽у厜鑶滅數浣?/span>鎴愬儚鍦?/span>鏃剁┖鍒嗚鲸鐜囥�佹祴閲忛�氶噺绛夋柟闈㈠叿鏈夋槑鏄剧殑浼樺娍銆傚叾涓紝鍙戝皠娉㈤暱鍦?/span>杩滅孩鍖猴紙640 nm浠ヤ笂锛夌殑鑽у厜鎺㈤拡鐢变簬鍏剁孩绉荤殑鍏夎氨鍏锋湁鏇村己缁勭粐绌块�忚兘鍔涳紝鑰屼笖鍙?/span>閫備簬澶?/span>閫氳矾鎴愬儚瑙傛祴锛屽洜鑰屽鍙?/span>鐮旂┒浜哄憳闈掔潗銆?/span>鐒惰�岀洰鍓嶅彲浣跨敤鐨?/span>杩滅孩鍖?/span>鑶滅數浣嶆帰閽?/span>鍦?/span>浜害鍜岀伒鏁忓害鏂归潰瀛樺湪涓ラ噸缂洪櫡锛屽洜姝?/span>浜熼渶鍙戝睍閫傜敤浜庤褰曠缁忓厓鍔ㄤ綔鐢典綅鐨勯珮鎬ц兘鑽у厜鎺㈤拡銆?/span>
2021骞?/span>4鏈?/span>15鏃?/span>锛屽寳浜ぇ瀛﹀寲瀛︿笌鍒嗗瓙宸ョ▼瀛﹂櫌閭归箯璇鹃缁勪笌闄堥箯璇鹃缁?/span>鍏卞悓鍦?/span>Nature Chemistry鏉傚織鍦ㄧ嚎鍙戣〃浜嗕粬浠渶鏂扮殑鐮旂┒鎴愭灉鈥?/span>A far-red hybrid voltage indicator enabled by bioorthogonal engineering of rhodopsin on live neurons鈥?/span>銆?/span>浠栦滑缁煎悎鍒╃敤鐢熺墿姝ｄ氦鍙嶅簲鍜岃啘铔嬬櫧宸ョ▼鍖?/span>鏀归�犵瓥鐣ワ紝寮�鍙?/span>鍑?/span>涓�绯诲垪鍏锋湁楂樼伒鏁忓害鍜屾垚鍍忎俊鍣瘮鐨?/span>鑽у厜鑶滅數浣嶆帰閽?/span>HVI锛?/span>hybrid voltage indicator锛?/span>銆?/span>鏍规嵁鎴愬儚鍏夎氨闇�姹傦紝HVI鐨勮泲鐧?/span>璐?/span>楠ㄦ灦鍙?/span>鍊熷姪鐢熺墿姝ｄ氦鍙嶅簲鎼厤涓嶅悓鍖栧缁撴瀯鐨勮崸鍏夋煋鏂欙紝鏋勫缓鍑轰竴绯诲垪璺ㄨ秺鍙鍏夎氨鐨?/span>澶嶅悎鍨嬫帰閽堛�傚叾涓紝姗欑孩鍖烘帰閽?/span>HVI-Cy3鍏锋湁鏈�楂樼殑鐏垫晱搴︼紝鑳藉浠ラ珮杈?/span>90鐨勪俊鍣瘮鎴愬儚璁板綍绁炵粡鍔ㄤ綔鐢典綅锛?/span>杩滅孩鍖?/span>鎺㈤拡HVI-Cy5鍏锋湁鏈�绾㈢Щ鐨勫厜璋憋紝涓嶄粎鍙笌缁胯壊鎴栫孩鑹?/span>鑽у厜鎺㈤拡鍚屾椂浣跨敤锛屽疄鐜拌啘鐢典綅涓?/span>閽?/span>绂诲瓙銆佺缁忛�掕川绛?/span>閲嶈鐢熺悊淇″彿鐨勫苟琛岃娴嬶紝杩樿兘澶?/span>涓庡厜閬椾紶瀛﹀伐鍏疯仈鐢?/span>锛?/span>瀹炵幇鍏ㄥ厜瀛?/span>绁炵粡鐢电敓鐞嗘娴嬨�?/span>
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閭归箯璇鹃缁勯暱鏈熻嚧鍔涗簬鍙戝睍鍜屽簲鐢ㄥ寲瀛︽帰閽堟妧鏈紝鐮旂┒鍙備笌绁炵粡淇″彿杞杩囩▼鐨勭敓鐗╁ぇ鍒嗗瓙銆佹敮閰嶇缁忔椿鍔ㄧ殑鍖栧鍜岀墿鐞嗕俊鍙枫�備粬浠巼鍏堟彁鍑?/span>鈥滃鍚堝瀷鑶滅數浣嶆帰閽堚�濈殑姒傚康锛?/span>鍒╃敤鍖栧鎵嬫灏嗚崸鍏夋煋鏂欎笌瑙嗙传绾㈣川铔嬬櫧鐩?/span>鍋惰仈锛?/span>鍒╃敤鍚庤�呯殑鐢佃嚧鍙樿壊鏁堝簲瀹炵幇鑶滅數浣嶆垚鍍?/span>锛?/span>Angew. Chem. Int. Ed. 2018, 57, 3949-3953锛夈�?/span>闄堥箯璇鹃缁勯暱鏈熻嚧鍔涗簬鍙戝睍閫傜敤浜庢椿缁嗚優鍙婃椿浣撳姩鐗?/span>鐨勭敓鐗╂浜ゅ弽搴旓紝骞?/span>閫氳繃閬椾紶缂栫爜鎶�鏈紝瀹炵幇浜嗚泲鐧借川鐨勭壒寮傛爣璁般�佹縺娲讳笌璋冩帶锛?/span>Nat. Chem. Biol. 2016, 12, 129-137锛?/span>銆?/span>鍦ㄦ渶鏂扮殑鐮旂┒鎴愭灉涓紝涓や釜璇鹃缁?/span>鍚堜綔锛屽皢鍖栧鍙嶅簲绛栫暐涓庤泲鐧?/span>璐?/span>楠ㄦ灦鏀归�?/span>鐩哥粨鍚?/span>锛屽绁炵粡鍏冪殑鑶滆泲鐧借繘琛屼簡鍘熶綅鐨?/span>鈥滅敓鐗╂浜も�濆伐绋嬩紭鍖?/span>銆?/span>涓�鏂归潰锛?/span>閴翠簬鐩墠澶у鏁扮敓鐗╂浜ゅ弽搴旈毦浠ラ珮鏁堝湴鏍囪鑶滆泲鐧斤紝浠ュ強鐐瑰嚮鍖栧鍙嶅簲锛堥摐鍌寲鐐斿熀-鍙犳爱鐜姞鎴愬弽搴旓級瀵?/span>绁炵粡鍏?/span>鐨勬瘨鎬?/span>锛?/span>浠栦滑鏀?/span>鐢?/span>浜?/span>鐢熺墿鐩稿鎬ф洿濂?/span>銆佹晥鐜囨洿楂?/span>鐨勯�嗙數瀛愰渶姹?/span>鐙勫皵鏂?/span>-闃垮皵寰?/span>鍙嶅簲锛?/span>IEDDA锛夛紝灏嗛珮鑽у厜閲忓瓙浜х巼鐨?/span>杩滅孩鍖?/span>鏌撴枡寮曞叆宸ョ▼鏀归�犵殑瑙嗙传绾㈣川铔嬬櫧鐨勭壒寮備綅鐐?/span>銆?/span>鍙︿竴鏂归潰锛?/span>浠栦滑閽堝瑙嗙传绾㈣川铔嬬櫧Ace2涓殑鍏抽敭璐ㄥ瓙鍙椾綋姘ㄥ熀閰告畫鍩?/span>杩涜绐佸彉绛涢�?/span>锛屾渶缁堝緱鍒颁簡娑堥櫎绋虫�佸厜鐢垫祦涓?/span>浜害鍙婄伒鏁忓害鏄捐憲鎻愬崌鐨勮繙绾㈠尯澶嶅悎鍨嬫帰閽?/span>HVI-Cy5銆?/span>褰撶缁忓厓鑶滅數鍔垮彂鐢?/span>鍘绘瀬鍖栨椂锛岃川瀛愮數鍖栧鍔?/span>鐨勬敼鍙樹績杩涜榛勯啗甯か纰辩殑璐ㄥ瓙鍖?/span>锛屼粠鑰屾敼鍙樿绱孩璐ㄧ殑鍚告敹鍏夎氨锛屾渶缁堥�氳繃FRET鏁堝簲褰卞搷鍏跺伓鑱旇崸鍏夋煋鏂欑殑閲忓瓙浜х巼锛屽鑷磋崸鍏変俊鍙峰彉鍖?/span>锛堝浘2锛?/span>銆?/span>
鍥?/span>2澶嶅悎鍨嬭啘鐢典綅鎺㈤拡HVI鑽у厜鏍囪鏂规硶绀烘剰鍥惧強鎺㈤拡鍝嶅簲缁嗚優鑶滅數浣嶅彉鍖栧師鐞?/span>鍥?/span>
瀹為獙琛ㄦ槑锛?/span>HVI-Cy5鍙笌鍏夐仐浼犲宸ュ叿鑱旂敤锛堝浘3锛?/span>銆傜爺绌朵汉鍛?/span>鐢ㄧ煭娉㈤暱鍏?/span>鍗曞悜鎴栬�呭弻鍚戣皟鎺?/span>绁炵粡鍏冨叴濂嬫�э紝鍚屾椂鍦?/span>杩滅孩鍖?/span>鑽у厜閫氶亾璁板綍鑶滅數浣嶅彉鍖?/span>锛?/span>鎵╁厖浜嗗叏鍏夊鐢电敓鐞嗗宸ュ叿绠?/span>锛堝浘3a-c锛?/span>銆?/span>璇ユ妧鏈浉姣斾簬浼犵粺鐨勫鐢垫瀬鑶滅墖閽冲埡婵�骞惰褰曪紝鎶�鏈毦搴︽槑鏄句笅闄嶃�?/span>HVI-Cy5杩樺彲浠ヤ笌鍏朵粬鑽у厜鎺㈤拡杩涜鍙岃壊鎴愬儚锛屾棤涓叉壈鐩戞祴缁嗚優鑶滅數鍔垮強閽欑瀛愩�侀�掕川鍜?/span>pH绛夌敓鐞嗕俊鍙?/span>锛堝浘3d-f锛?/span>銆傚鑹叉垚鍍忓皢甯姪鐮旂┒鑰呮洿濂藉湴浜嗚В鑶滅數浣嶄笌鍏朵粬鐢熺悊淇″彿鍔ㄦ�佸彉鍖栫殑鑱旂郴鍜屽樊寮傘�?/span>
鍥?/span>3鍏锋湁娣辩孩鑽у厜鍏夎氨鐨?/span>HVI-Cy5鍙笌鍏夐仐浼犲宸ュ叿鍙婇挋鎺㈤拡鑱旂敤瀹炵幇鍏ㄥ厜瀛︾數鐢熺悊妫�娴?/span>
姝ゅ锛?/span>瀹為獙灏?/span>HVI-Cy5涓?/span>鍏夐仐浼犲宸ュ叿鍒嗗埆琛ㄨ揪鍦?/span>涓嶅悓鐨勫ぇ榧犳捣椹綋绁炵粡鍏?/span>涓?/span>锛?/span>鍒╃敤鑶滅數浣?/span>鎴愬儚璇勪及APV鍜?/span>NBQX涓ょ鑽墿瀵圭缁忓厓绐佽Е杩炴帴鐨?/span>褰卞搷锛?/span>瑙ｆ瀽浜?/span>NMDAR鍜?/span>AMPAR涓?/span>绫?/span>璋锋皑閰稿彈浣撳绐佽Е淇″彿浼犻�掔殑璐＄尞锛?/span>鍥?/span>4锛?/span>銆?/span>鏈潵锛?/span>HVI-Cy5鏈?/span>鏈?/span>鐢ㄤ簬鍦?/span>浣撳绛涢�変綔鐢ㄤ簬鍙椾綋铔嬬櫧鐨勬縺鍔ㄥ墏鎴栨嫯鎶楀墏銆?/span>
鍥?/span>4 灏?/span>HVI-Cy5涓庡厜閬椾紶瀛﹀伐鍏峰垎鍒〃杈惧湪涓嶅悓绁炵粡鍏冧腑鍙?/span>浠ョ爺绌惰嵂鐗╁绁炵粡绐佽Е鐨勪綔鐢?/span>
鎬讳箣锛?/span>鏈枃閫氳繃瀵硅绱孩璐ㄨ啘铔嬬櫧鐨勨�滅敓鐗╂浜も�濆伐绋嬩紭鍖栵紝鍙戝睍浜嗛�傜敤浜?/span>绁炵粡鍏冪數淇″彿璁板綍鐨?/span>杩滅孩鍖?/span>楂樻�ц兘鑽у厜鎺㈤拡HVI-Cy5锛?/span>瀹炵幇澶氳壊鎴愬儚鍜屽叏鍏夊鐢电敓鐞嗗搴旂敤锛屾湡寰呰鎺㈤拡鑳藉甯姪鐮旂┒鑰呰В璇绘洿鍔犲鏉傜殑绁炵粡鍏冪數鐢熺悊淇″彿銆?/span>
鍖椾含澶у鍖栧涓庡垎瀛愬伐绋嬪闄㈠崥澹爺绌剁敓鍒樹功褰般�佹灄鐣咃紝鍖楀ぇ-娓呭崕鐢熷懡鑱斿悎涓績鍗氬＋姣曚笟鐢熻儱姘告樉锛堢幇娴欐睙澶у鍖诲涓績鍗氬＋鍚庯級涓鸿璁烘枃鐨勫叡鍚岀涓�浣滆�呫�?/span>鍖椾含澶у鍖栧涓庡垎瀛愬伐绋嬪闄?/span>閭归箯鐮旂┒鍛樺拰闄堥箯鏁欐巿涓鸿璁烘枃鐨勫叡鍚岄�氳浣滆�呫�?/span>璇ュ伐浣滃緱鍒颁簡鍥藉鑷劧绉戝鍩洪噾濮斻�佺鎶�閮ㄣ�佺敓鐗╂湁鏈轰笌鍒嗗瓙宸ョ▼鏁欒偛閮ㄩ噸鐐瑰疄楠屽銆佸寳浜垎瀛愮瀛﹀浗瀹剁爺绌朵腑蹇冨拰鍖楀ぇ-娓呭崕鐢熷懡绉戝鑱斿悎涓績鐨勮祫鍔┿�?/span>
鍘熸枃閾炬帴锛?/span>//www.nature.com/articles/s41557-021-00641-1