瀹為獙瀹ゆ柊闂?/h4>
璐炬鑺宠棰樼粍鎶ラ亾妞嶇墿m6A缁撳悎铔嬬櫧鍦ㄨ仛鑵鸿嫹閰稿寲杩囩▼涓殑璋冩帶鍔熻兘
杩?/span>鏃ワ紝鍖椾含澶у鍖栧涓庡垎瀛愬伐绋嬪闄?/span>璐炬鑺?/span>璇鹃缁勫湪 銆?/span>Molecular Plant銆?/span> 涓?/span>鍦ㄧ嚎鍙戣〃浜嗛涓?/span> 鈥?/span>Arabidopsis N6-methyladenosine reader CPSF30-L recognizes FUE signal to control polyadenylation site choice in liquid-like nuclear body鈥?/span> 鐨勭爺绌惰鏂?/span>銆傝鐮旂┒閴村畾浜?/span>CPSF30-L涓烘鐗╂嫙鍗?/span>鑺?/span>涓殑涓�涓叏鏂?/span>m6A缁撳悎铔嬬櫧锛?/span>鎻ず浜?/span>CPSF30-L渚濊禆m6A璇嗗埆淇冭繘鐩?/span>鍙?/span>杩囩▼锛屼互鍙?/span>璋冩帶閫夋嫨鎬у鑱氳吅鑻烽吀鍖?/span>鐨勫垎瀛愭満鍒?/span>銆?/span>
m6A鏄?/span>mRNA涓?/span>涓板害鏈�楂?/span>鐨?/span>鍖栧淇グ锛?/span>鏅亶瀛樺湪浜庡姩鐗┿�佹鐗?/span>銆佺湡鑿屼互鍙?/span>RNA鐥呮瘨涔嬩腑銆?/span>m6A鍙互鍒嗗埆鍦ㄧ敳鍩鸿浆绉婚叾澶嶅悎鐗╁拰鍘荤敳鍩洪叾鐨勪綔鐢ㄤ笅琚?/span>鍙�嗗湴娣诲姞鍜?/span>鍘婚櫎锛?/span>骞?/span>鐢?/span>m6A缁撳悎铔嬬櫧璇嗗埆骞?/span>褰卞搷RNA浠ｈ阿鐨勫悇涓柟闈?/span>銆?/span>灏界m6A鐩稿叧铔嬬櫧鍦ㄥ摵涔冲姩鐗╀腑宸茬粡琚厖鍒嗗湴閴村畾鍙婅〃寰侊紝浣?/span>m6A鍦?/span>妞嶇墿鐢熼暱鍙戣偛涓?/span>浠ュ強RNA浠ｈ阿涓?/span>鐨?/span>璋冩帶鍔熻兘鐮旂┒浠?/span>鍗佸垎鏈夐檺銆?/span>
璐炬鑺?/span>璇鹃缁?/span>鑷村姏浜庣爺绌?/span>m6A鍦ㄦ鐗╀綋涓奖鍝嶇敓闀垮彂鑲蹭互鍙婂埡婵�鍝嶅簲鐨勫垎瀛愭満鍒讹紝鍦ㄦ鍓嶇殑鐮旂┒涓紝璇ヨ棰樼粍缁樺埗浜嗘鐗╂嫙鍗?/span>鑺?/span>鍜岀帀绫崇殑RNA淇グm6A鍏ㄨ浆褰?/span>鍥捐氨锛?/span>Nature Communications, 2014, 5, 5630锛?/span>Plant Physiology, 2020, 182, 332锛夛紝閴村畾浜嗘鐗╂嫙鍗?/span>鑺?/span>涓?/span>m6A鍘讳慨楗伴叾ALKBH10B鍜?/span>绗竴涓?/span>m6A缁撳悎铔嬬櫧ECT2鐨勭敓鐗╁姛鑳?/span>锛?/span>Plant Cell, 2018, 30, 968锛?/span>Plant Cell, 2017, 29, 2995锛?/span>锛屾帹鍔ㄤ簡妞嶇墿涓〃瑙?/span>杞綍缁?/span>瀛︾殑鐮旂┒銆?/span>涓嶅悓鐨?/span>m6A璇嗗埆铔嬬櫧鍦?/span>mRNA鐨勪唬璋㈣繃绋嬩腑鍙戞尌鐫�涓嶅悓鐨勮皟鎺у姛鑳?/span>锛?/span> m6A缁撳悎缁撴瀯鍩?/span>YTH鍦ㄦ嫙鍗?/span>鑺?/span>涓湁13绉嶅悓婧愯泲鐧戒箣澶氾紝鐒惰�屽浜庢嫙鍗?/span>鑺?/span>涓?/span>YTH缁撳悎瀹舵棌铔嬬櫧鐨勭爺绌朵互鍙?/span>璋冩帶鏈哄埗鐩稿杈冨皯銆?/span>
璇ョ爺绌堕壌瀹氬嚭鎷熷崡鑺?/span>涓?/span>CPSF30-L涓?/span>m6A鐨勭粨鍚堣泲鐧斤紝骞朵笖鍙戠幇CPSF30-L鍙互閫氳繃m6A鐨勭粨鍚堣兘鍔?/span>褰卞搷鎷熷崡鑺?/span>鐨勫紑鑺变互鍙?/span>ABA鍝嶅簲銆?/span>浜氱粏鑳炲畾浣嶄互鍙?/span>FRAP瀹為獙琛ㄦ槑CPSF30-L铔嬬櫧鍒嗗竷浜庣粏鑳炴牳涓苟鍙戠敓娑叉恫鐩稿垎绂诲舰鎴愭牳浣撶粨鏋勶紝骞朵笖CPSF30-L鐨?/span>m6A缁撳悎鍔熻兘鍙互淇冭繘鐩稿垎绂荤殑鏍镐綋褰㈡垚銆?/span>
鍒╃敤璇ヨ棰樼粍涔嬪墠寮�鍙?/span>鐨?/span>鐢查啗浜よ仈-鍏嶇柅鍏辨矇娣�鎶�鏈紝鍦?/span>鍏?/span>杞綍缁?/span>姘村钩涓婇壌瀹氫簡CPSF30-L-RNA鐩镐簰浣滅敤浣嶇偣锛屾彮绀?/span>浜?/span>CPSF30-L涓昏缁撳悎mRNA鐨?/span>3'闈炵炕璇戝尯 (3'UTR),骞朵笖鍊惧悜浜庣粨鍚?/span>淇濆畧m6A鍩哄簭浠ュ強鑱氳吅鑻烽吀鍖栦俊鍙?/span>鈥?/span>UGUA銆?/span>GAAMH銆?/span>AAUAAA锛屾殫绀?/span>CPSF30-L閫氳繃璇嗗埆m6A淇グ鐨勮仛鑵鸿嫹閰稿寲淇″彿璋冩帶pre-mRNA 3'鏈鐨勫姞宸ヨ繃绋?/span>銆?/span>闅忓悗锛屽閲庣敓鍨嬪拰CPSF30-L鐨?/span>T-DNA鎻掑叆绐佸彉浣?/span>cpsf30-l杩涜poly(A)浣嶇偣娴嬪簭鍒嗘瀽锛?/span>鍙戠幇CPSF30-L鐨勭牬鍧忎細鏁翠綋褰卞搷poly锛?/span>A锛変綅鐐圭殑閫夋嫨锛岃繘涓�姝ョ殑鍒嗘瀽璇佸疄锛?/span>CPSF30-L閫氳繃璇嗗埆 m6A淇グ鐨?/span>FUE鑱氳吅鑻烽吀鍖栦俊鍙疯繘鑰?/span>绮惧瘑鎺у埗poly锛?/span>A锛変綅鐐圭殑閫夋嫨銆?/span>
涓?/span>鎷熷崡鑺?/span>寮�鑺变互鍙?/span>ABA鍝嶅簲鐨勪笁涓熀鍥?/span>SOC1, RPN10鍙?/span>FYVE1鐨勮浆褰曟湰鍙互琚?/span>m6A淇グ涓旇CPSF30-L缁撳悎銆傜浉瀵逛簬閲庣敓鍨嬭�岃█锛?/span>鍦?/span>cpsf30-l涓紝SOC1, RPN10鍙?/span>FYVE1鐨?/span>pre-mRNA鍦?/span>3鈥?/span>UTR鍔犲伐杩囩▼涓洿鍊惧悜浜庤繙绔?/span>poly(A)浣嶇偣锛?/span>瀵艰嚧3鈥?/span>UTR鍔犻暱鏄撹闄嶈В锛?/span>鐩稿簲mRNA琛ㄨ揪閲?/span>涓嬮檷锛岀户鑰屽鑷?/span>cpsf30-l绐佸彉浣撲腑鍛堢幇鍑烘櫄鑺变互鍙?/span>ABA鍝嶅簲鏁忔劅鐨勮〃鍨?/span>锛堝浘涓�锛?/span>銆?/span>杩欓」鐮旂┒鍙戠幇浜嗘鐗╀腑m6A鐨勫叏鏂板姛鑳?/span>鈥?/span>璋冩帶妞嶇墿pre-mRNA鑱氳吅鑻烽吀鍖栧拰妞嶇墿鐩稿垎绂荤殑鍒嗗瓙鏈哄埗銆?/span>
鍥句竴. CPSF30-L 璋冩帶鑱氳吅鑻烽吀鍖栫殑鍒嗗瓙鏈哄埗鍥?/span>
鍖椾含澶у鍖栧涓庡垎瀛愬伐绋嬪闄㈣淳妗傝姵鐮旂┒鍛?/span>涓鸿璁烘枃鐨勯�氳浣滆�咃紝2017绾?/span>鍗氬＋鐮旂┒鐢?/span>鐢熷畫鍩瑰摬锛屽崥澹悗鏉ㄥ啗娉?/span>涓鸿璁烘枃鐨?/span>鍏卞悓绗竴浣滆�?/span>锛?/span>璇ョ爺绌跺緱鍒颁簡绉戞妧閮ㄣ�佸浗瀹惰嚜鐒剁瀛﹀熀閲戝銆佸寳浜ぇ瀛﹀強鍖椾含鍒嗗瓙绉戝鍥藉鐮旂┒涓績鐨?/span>璧勫姪銆?/span>
鏂囩珷閾炬帴: //www.sciencedirect.com/science/article/pii/S1674205221000149